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Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w
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Th Freund's adjuvant. The first does was in Freund's complete adjuvant (Sigma), and subsequent injections were given in Freund's incomplete adjuvant. Set II was immunized with gHt-gL mixed with an equal volume of alum adjuvant (Pierce). Virus neutralization assay. Rabbit or mouse sera were treated at 56 for 30 min to inactivate complement. Serial twofold dilutions of serum were prepared in DMEM c
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