Polymerase chain reaction (PCR) was performed to verify the presence of the putative zebrafish kcnj2 orthologous genes. All primers are listed in Supplementary Table 2. PCR and 3 RACE methods were performed as previously described [24]. The amplicons were introduced into the pGEM T-Easy vector (Promega) according to the manufacturer's instructions, and the products were bidirectionally sequenced t
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