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Rotein G surfaces, according to their isotype, at a flow rate of 2 mL/min for 4 min injection. The analyte BP-2a 515 variant in 2-fold serial dilutions in running buffer (starting from 500 or 250 nM, five concentrations in total) was injected over the captured antibody for 2 min at 45 mL/min followed by a 5 or 10 min dissociation. Biosensor regeneration was performed after each cycle and achieved
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