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Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
1
E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
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E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
1
Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal
1
T ambient temperature has limited or no impact on respiratory virus detection by PCR [14,20,21], although investigating further the effects of transporting samples for extended periods and at higher temperatures was highlighted in one study [20]. The observational research in childhood infectious diseases (ORChID) project is a longitudinal, communitybased, dynamic birth cohort study, which seeks t
1
E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
1
E validity of real-time PCR results for respiratory virus investigations in community-based studies. Keywords: Nasal swab, Respiratory virus, Real-time polymerase chain reaction, Quality control, Mould, Community-based study* Correspondence: a.alsaleh@uq.edu.au 1 Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia 2 Queensland Paediat
1
Ssical culture and phenotypic-based methods and have led to the discovery of new respiratory viruses [2]. However, contemporary studies employing these new techniques have often used convenience samples obtained from patients admitted to hospital or attending Emergency Department clinics [3-5]. Understanding more fully the ARI disease burden in the community is important for developing public heal